The regiment was formed as the Royal Highland Fusiliers (Princess Margaret's Own Glasgow and Ayrshire Regiment) on 20 January 1959 by the amalgamation of the Royal Scots Fusiliers with the Highland Light Infantry (City of Glasgow Regiment). The Royal Highland Fusiliers, abbreviated as 'The RHF', were part of the Scottish Division.
In 2006 the regiment was amalgamated with the other regiments of the Scottish Division to become the 2nd Battalion of the Royal Regiment of Scotland.